Construct: shRNA TRCN0000338646

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001014283.1-383s21c1
DNA Barcode:
GTGAATTTAGCAGAAAGGAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
DCUN1D2 (55208)
Gene Description:
defective in cullin neddylation 1 domain containing 2
Transcript:
RefSeq NM_001014283.1 (NON-CURRENT)
Match location:
Position 383 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000338646)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTGAATTTAGCAGAAAGGAAT
Hairpin Sequence:
5'-CCGG-GTGAATTTAGCAGAAAGGAAT-CTCGAG-ATTCCTTTCTGCTAAATTCAC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTGAATTTAGCAGAAAGGAATCTCGAGATTCCTTTCTGCTAAATTCACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTGAATTTAGCAGAAAGGAATCTCGAGATTCCTTTCTGCTAAATTCAC-3'

Other clones with same target sequence:

  1. TRCN0000121884