Construct: shRNA TRCN0000121884

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_018185.1-366s1c1
DNA Barcode:
GTGAATTTAGCAGAAAGGAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
DCUN1D2 (55208)
Gene Description:
defective in cullin neddylation 1 domain containing 2
Transcript:
RefSeq NM_018185.1 (NON-CURRENT)
Match location:
Position 366 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000121884)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTGAATTTAGCAGAAAGGAAT
Hairpin Sequence:
5'-CCGG-GTGAATTTAGCAGAAAGGAAT-CTCGAG-ATTCCTTTCTGCTAAATTCAC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTGAATTTAGCAGAAAGGAATCTCGAGATTCCTTTCTGCTAAATTCACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTGAATTTAGCAGAAAGGAATCTCGAGATTCCTTTCTGCTAAATTCAC-3'

Other clones with same target sequence:

  1. TRCN0000338646