Construct: shRNA TRCN0000339194

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_134123.3-2542s21c1
DNA Barcode:
CCGGTAGTACCAACATCTTTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Scaf8 (106583)
Gene Description:
SR-related CTD-associated factor 8
Transcript:
RefSeq NM_134123.3 (CURRENT)
Match location:
Position 2542 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000339194)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCGGTAGTACCAACATCTTTA
Hairpin Sequence:
5'-CCGG-CCGGTAGTACCAACATCTTTA-CTCGAG-TAAAGATGTTGGTACTACCGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCGGTAGTACCAACATCTTTACTCGAGTAAAGATGTTGGTACTACCGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCGGTAGTACCAACATCTTTACTCGAGTAAAGATGTTGGTACTACCGG-3'

Other clones with same target sequence:

(none)