Construct: shRNA TRCN0000339265

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_134123.3-4540s21c1
DNA Barcode:
GGGTATGGCAATGACATATTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Scaf8 (106583)
Gene Description:
SR-related CTD-associated factor 8
Transcript:
RefSeq NM_134123.3 (CURRENT)
Match location:
Position 4540 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000339265)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GGGTATGGCAATGACATATTT
Hairpin Sequence:
5'-CCGG-GGGTATGGCAATGACATATTT-CTCGAG-AAATATGTCATTGCCATACCC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGGGTATGGCAATGACATATTTCTCGAGAAATATGTCATTGCCATACCCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGGGTATGGCAATGACATATTTCTCGAGAAATATGTCATTGCCATACCC-3'

Other clones with same target sequence:

(none)