Construct: shRNA TRCN0000339368

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_011206.2-637s21c1
DNA Barcode:
GTGCACCAGCTACAGTATATG

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ptpn18 (19253)
Gene Description:
protein tyrosine phosphatase, non-receptor type 18
Transcript:
RefSeq NM_011206.2 (CURRENT)
Match location:
Position 637 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000339368)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTGCACCAGCTACAGTATATG
Hairpin Sequence:
5'-CCGG-GTGCACCAGCTACAGTATATG-CTCGAG-CATATACTGTAGCTGGTGCAC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTGCACCAGCTACAGTATATGCTCGAGCATATACTGTAGCTGGTGCACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTGCACCAGCTACAGTATATGCTCGAGCATATACTGTAGCTGGTGCAC-3'

Other clones with same target sequence:

(none)