Construct: shRNA TRCN0000339508

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_172275.2-820s21c1
DNA Barcode:
CGCACACTTGGACTTCATGTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Trafd1 (231712)
Gene Description:
TRAF type zinc finger domain containing 1
Transcript:
RefSeq NM_172275.2 (CURRENT)
Match location:
Position 820 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000339508)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGCACACTTGGACTTCATGTT
Hairpin Sequence:
5'-CCGG-CGCACACTTGGACTTCATGTT-CTCGAG-AACATGAAGTCCAAGTGTGCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGCACACTTGGACTTCATGTTCTCGAGAACATGAAGTCCAAGTGTGCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGCACACTTGGACTTCATGTTCTCGAGAACATGAAGTCCAAGTGTGCG-3'

Other clones with same target sequence:

  1. TRCN0000181475