Construct: shRNA TRCN0000339531

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_153157.2-489s21c1
DNA Barcode:
GATGATCGGAAGACGCTAATG

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Olfm3 (229759)
Gene Description:
olfactomedin 3
Transcript:
RefSeq NM_153157.2 (NON-CURRENT)
Match location:
Position 489 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000339531)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GATGATCGGAAGACGCTAATG
Hairpin Sequence:
5'-CCGG-GATGATCGGAAGACGCTAATG-CTCGAG-CATTAGCGTCTTCCGATCATC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGATGATCGGAAGACGCTAATGCTCGAGCATTAGCGTCTTCCGATCATCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGATGATCGGAAGACGCTAATGCTCGAGCATTAGCGTCTTCCGATCATC-3'

Other clones with same target sequence:

(none)