Construct: shRNA TRCN0000339790

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_027891.4-954s21c1
DNA Barcode:
CCTGCAGTGTCATAGCAGAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Lrwd1 (71735)
Gene Description:
leucine-rich repeats and WD repeat domain containing 1
Transcript:
RefSeq NM_027891.4 (CURRENT)
Match location:
Position 954 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000339790)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTGCAGTGTCATAGCAGAAA
Hairpin Sequence:
5'-CCGG-CCTGCAGTGTCATAGCAGAAA-CTCGAG-TTTCTGCTATGACACTGCAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTGCAGTGTCATAGCAGAAACTCGAGTTTCTGCTATGACACTGCAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTGCAGTGTCATAGCAGAAACTCGAGTTTCTGCTATGACACTGCAGG-3'

Other clones with same target sequence:

  1. TRCN0000119912