Construct: shRNA TRCN0000339957

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_053227.2-252s21c1
DNA Barcode:
ATGCCAATCCCTTATCTATTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Vmn1r44 (113854)
Gene Description:
vomeronasal 1 receptor 44
Transcript:
RefSeq NM_053227.2 (CURRENT)
Match location:
Position 252 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000339957)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ATGCCAATCCCTTATCTATTT
Hairpin Sequence:
5'-CCGG-ATGCCAATCCCTTATCTATTT-CTCGAG-AAATAGATAAGGGATTGGCAT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGATGCCAATCCCTTATCTATTTCTCGAGAAATAGATAAGGGATTGGCATTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAATGCCAATCCCTTATCTATTTCTCGAGAAATAGATAAGGGATTGGCAT-3'

Other clones with same target sequence:

(none)