Construct: shRNA TRCN0000340374

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_172513.3-445s21c1
DNA Barcode:
TAGCTCTGAAGTTCGACTTAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Fam126b (213056)
Gene Description:
family with sequence similarity 126, member B
Transcript:
RefSeq NM_172513.3 (CURRENT)
Match location:
Position 445 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000340374)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TAGCTCTGAAGTTCGACTTAA
Hairpin Sequence:
5'-CCGG-TAGCTCTGAAGTTCGACTTAA-CTCGAG-TTAAGTCGAACTTCAGAGCTA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTAGCTCTGAAGTTCGACTTAACTCGAGTTAAGTCGAACTTCAGAGCTATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATAGCTCTGAAGTTCGACTTAACTCGAGTTAAGTCGAACTTCAGAGCTA-3'

Other clones with same target sequence:

(none)