Construct: shRNA TRCN0000340431

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_030087.2-529s21c1
DNA Barcode:
CACGTGCAGAAAGTGACAAAC

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ndufv3 (78330)
Gene Description:
NADH dehydrogenase (ubiquinone) flavoprotein 3
Transcript:
RefSeq NM_030087.2 (CURRENT)
Match location:
Position 529 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000340431)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CACGTGCAGAAAGTGACAAAC
Hairpin Sequence:
5'-CCGG-CACGTGCAGAAAGTGACAAAC-CTCGAG-GTTTGTCACTTTCTGCACGTG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCACGTGCAGAAAGTGACAAACCTCGAGGTTTGTCACTTTCTGCACGTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACACGTGCAGAAAGTGACAAACCTCGAGGTTTGTCACTTTCTGCACGTG-3'

Other clones with same target sequence:

(none)