Construct: shRNA TRCN0000340589

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_130448.3-575s21c1
DNA Barcode:
ATCGGTAATTGCTAGACTATC

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Pcdh18 (73173)
Gene Description:
protocadherin 18
Transcript:
RefSeq NM_130448.3 (CURRENT)
Match location:
Position 575 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000340589)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ATCGGTAATTGCTAGACTATC
Hairpin Sequence:
5'-CCGG-ATCGGTAATTGCTAGACTATC-CTCGAG-GATAGTCTAGCAATTACCGAT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGATCGGTAATTGCTAGACTATCCTCGAGGATAGTCTAGCAATTACCGATTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAATCGGTAATTGCTAGACTATCCTCGAGGATAGTCTAGCAATTACCGAT-3'

Other clones with same target sequence:

(none)