Construct: shRNA TRCN0000340981

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_028194.2-6485s21c1
DNA Barcode:
ATAACGGATGGACGGATAAAC

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Fryl (72313)
Gene Description:
FRY like transcription coactivator
Transcript:
RefSeq NM_028194.2 (CURRENT)
Match location:
Position 6485 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000340981)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ATAACGGATGGACGGATAAAC
Hairpin Sequence:
5'-CCGG-ATAACGGATGGACGGATAAAC-CTCGAG-GTTTATCCGTCCATCCGTTAT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGATAACGGATGGACGGATAAACCTCGAGGTTTATCCGTCCATCCGTTATTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAATAACGGATGGACGGATAAACCTCGAGGTTTATCCGTCCATCCGTTAT-3'

Other clones with same target sequence:

(none)