Construct: shRNA TRCN0000341013

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_027930.3-1625s21c1
DNA Barcode:
GAGCAGAAGATGATATTGTTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Mtfr2 (71804)
Gene Description:
mitochondrial fission regulator 2
Transcript:
RefSeq NM_027930.3 (CURRENT)
Match location:
Position 1625 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000341013)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAGCAGAAGATGATATTGTTT
Hairpin Sequence:
5'-CCGG-GAGCAGAAGATGATATTGTTT-CTCGAG-AAACAATATCATCTTCTGCTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAGCAGAAGATGATATTGTTTCTCGAGAAACAATATCATCTTCTGCTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAGCAGAAGATGATATTGTTTCTCGAGAAACAATATCATCTTCTGCTC-3'

Other clones with same target sequence:

  1. TRCN0000191943