Construct: shRNA TRCN0000341033

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_013586.4-2172s21c1
DNA Barcode:
GGTCGTTATCAACCCAAATTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Loxl3 (16950)
Gene Description:
lysyl oxidase-like 3
Transcript:
RefSeq NM_013586.4 (CURRENT)
Match location:
Position 2172 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000341033)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GGTCGTTATCAACCCAAATTT
Hairpin Sequence:
5'-CCGG-GGTCGTTATCAACCCAAATTT-CTCGAG-AAATTTGGGTTGATAACGACC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGGTCGTTATCAACCCAAATTTCTCGAGAAATTTGGGTTGATAACGACCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGGTCGTTATCAACCCAAATTTCTCGAGAAATTTGGGTTGATAACGACC-3'

Other clones with same target sequence:

(none)