Construct: shRNA TRCN0000341105

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_013586.4-2704s21c1
DNA Barcode:
CCGCGTTACCTCTAGCCATTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Loxl3 (16950)
Gene Description:
lysyl oxidase-like 3
Transcript:
RefSeq NM_013586.4 (CURRENT)
Match location:
Position 2704 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000341105)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCGCGTTACCTCTAGCCATTT
Hairpin Sequence:
5'-CCGG-CCGCGTTACCTCTAGCCATTT-CTCGAG-AAATGGCTAGAGGTAACGCGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCGCGTTACCTCTAGCCATTTCTCGAGAAATGGCTAGAGGTAACGCGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCGCGTTACCTCTAGCCATTTCTCGAGAAATGGCTAGAGGTAACGCGG-3'

Other clones with same target sequence:

(none)