Construct: shRNA TRCN0000341172

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001162970.1-4250s21c1
DNA Barcode:
CAAACAGCGCCGAGCCTATTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Crybg2 (230806)
Gene Description:
crystallin beta-gamma domain containing 2
Transcript:
RefSeq NM_001162970.1 (CURRENT)
Match location:
Position 4250 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000341172)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAAACAGCGCCGAGCCTATTT
Hairpin Sequence:
5'-CCGG-CAAACAGCGCCGAGCCTATTT-CTCGAG-AAATAGGCTCGGCGCTGTTTG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAAACAGCGCCGAGCCTATTTCTCGAGAAATAGGCTCGGCGCTGTTTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAAACAGCGCCGAGCCTATTTCTCGAGAAATAGGCTCGGCGCTGTTTG-3'

Other clones with same target sequence:

(none)