Transcript: Mouse NM_001162970.1

Mus musculus crystallin beta-gamma domain containing 2 (Crybg2), mRNA.

Source:
NCBI, updated 2017-06-14
Taxon:
Mus musculus (mouse)
Gene:
Crybg2 (230806)
Length:
4781
CDS:
114..4664

Additional Resources:

NCBI RefSeq record:
NM_001162970.1
NBCI Gene record:
Crybg2 (230806)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse NM_001162970.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000341172 CAAACAGCGCCGAGCCTATTT pLKO_005 4250 CDS 100% 13.200 18.480 N Crybg2 n/a
2 TRCN0000341173 CTACTAGAAGAGGGCGAATAT pLKO_005 3366 CDS 100% 13.200 18.480 N Crybg2 n/a
3 TRCN0000341174 CCGAGGCTGCTGGATACTATA pLKO_005 2744 CDS 100% 13.200 10.560 N Crybg2 n/a
4 TRCN0000341241 TATCCTAGAGCCCGGAGAATA pLKO_005 3065 CDS 100% 13.200 10.560 N Crybg2 n/a
5 TRCN0000341175 ACTTTCTGGGCGACCACATTT pLKO_005 3772 CDS 100% 13.200 9.240 N Crybg2 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001162970.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_12147 pDONR223 100% 34.6% 35.4% None (many diffs) n/a
2 ccsbBroad304_12147 pLX_304 0% 34.6% 35.4% V5 (many diffs) n/a
3 TRCN0000481400 ACACTTGTGTCTAGTGCGGATGTA pLX_317 21.2% 34.6% 35.4% V5 (many diffs) n/a
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