Construct: shRNA TRCN0000341409

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_177375.1-994s21c1
DNA Barcode:
TGAGAATTGCCCGAGTCTATA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Rab26 (328778)
Gene Description:
RAB26, member RAS oncogene family
Transcript:
RefSeq NM_177375.1 (CURRENT)
Match location:
Position 994 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000341409)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGAGAATTGCCCGAGTCTATA
Hairpin Sequence:
5'-CCGG-TGAGAATTGCCCGAGTCTATA-CTCGAG-TATAGACTCGGGCAATTCTCA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGAGAATTGCCCGAGTCTATACTCGAGTATAGACTCGGGCAATTCTCATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGAGAATTGCCCGAGTCTATACTCGAGTATAGACTCGGGCAATTCTCA-3'

Other clones with same target sequence:

(none)