Construct: shRNA TRCN0000341615

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_030235.1-1147s21c1
DNA Barcode:
CAGTGATTCTGGCCGATATTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Avl9 (78937)
Gene Description:
AVL9 cell migration associated
Transcript:
RefSeq NM_030235.1 (CURRENT)
Match location:
Position 1147 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000341615)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAGTGATTCTGGCCGATATTT
Hairpin Sequence:
5'-CCGG-CAGTGATTCTGGCCGATATTT-CTCGAG-AAATATCGGCCAGAATCACTG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAGTGATTCTGGCCGATATTTCTCGAGAAATATCGGCCAGAATCACTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAGTGATTCTGGCCGATATTTCTCGAGAAATATCGGCCAGAATCACTG-3'

Other clones with same target sequence:

(none)