Construct: shRNA TRCN0000341657

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001146349.1-2306s21c1
DNA Barcode:
GTCACTTTCGTTAACTATATA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Rnf217 (268291)
Gene Description:
ring finger protein 217
Transcript:
RefSeq NM_001146349.1 (CURRENT)
Match location:
Position 2306 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000341657)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTCACTTTCGTTAACTATATA
Hairpin Sequence:
5'-CCGG-GTCACTTTCGTTAACTATATA-CTCGAG-TATATAGTTAACGAAAGTGAC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTCACTTTCGTTAACTATATACTCGAGTATATAGTTAACGAAAGTGACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTCACTTTCGTTAACTATATACTCGAGTATATAGTTAACGAAAGTGAC-3'

Other clones with same target sequence:

(none)