Transcript: Mouse NM_001146349.1

Mus musculus ring finger protein 217 (Rnf217), mRNA.

Source:
NCBI, updated 2017-04-22
Taxon:
Mus musculus (mouse)
Gene:
Rnf217 (268291)
Length:
2873
CDS:
542..2089

Additional Resources:

NCBI RefSeq record:
NM_001146349.1
NBCI Gene record:
Rnf217 (268291)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse NM_001146349.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000341590 AGCAGTTGTAATCGGTTTATT pLKO_005 2002 CDS 100% 15.000 21.000 N Rnf217 n/a
2 TRCN0000341592 CCTTGGCATGAAGGTGTTAAC pLKO_005 1625 CDS 100% 10.800 8.640 N Rnf217 n/a
3 TRCN0000341659 ACAACTGTTGTCTACAATTTA pLKO_005 1406 CDS 100% 15.000 10.500 N Rnf217 n/a
4 TRCN0000341658 CACATCAAACCTCAGTATATT pLKO_005 1855 CDS 100% 15.000 10.500 N Rnf217 n/a
5 TRCN0000341657 GTCACTTTCGTTAACTATATA pLKO_005 2306 3UTR 100% 15.000 10.500 N Rnf217 n/a
6 TRCN0000034127 CCACACATCAAACCTCAGTAT pLKO.1 1852 CDS 100% 4.950 3.465 N RNF217 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001146349.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_05079 pDONR223 100% 39.4% 41.3% None (many diffs) n/a
2 ccsbBroad304_05079 pLX_304 0% 39.4% 41.3% V5 (many diffs) n/a
3 TRCN0000476605 TACTTTCGTGGAAGTTTTCAATTG pLX_317 39.8% 39.4% 41.3% V5 (many diffs) n/a
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