Construct: shRNA TRCN0000341658

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001146349.1-1855s21c1
DNA Barcode:
CACATCAAACCTCAGTATATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Rnf217 (268291)
Gene Description:
ring finger protein 217
Transcript:
RefSeq NM_001146349.1 (CURRENT)
Match location:
Position 1855 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000341658)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CACATCAAACCTCAGTATATT
Hairpin Sequence:
5'-CCGG-CACATCAAACCTCAGTATATT-CTCGAG-AATATACTGAGGTTTGATGTG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCACATCAAACCTCAGTATATTCTCGAGAATATACTGAGGTTTGATGTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACACATCAAACCTCAGTATATTCTCGAGAATATACTGAGGTTTGATGTG-3'

Other clones with same target sequence:

(none)