Construct: shRNA TRCN0000341878

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_030071.1-623s21c1
DNA Barcode:
AGTATCGATGCTCCGTGTTTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Mroh9 (78258)
Gene Description:
maestro heat-like repeat family member 9
Transcript:
RefSeq NM_030071.1 (CURRENT)
Match location:
Position 623 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000341878)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGTATCGATGCTCCGTGTTTA
Hairpin Sequence:
5'-CCGG-AGTATCGATGCTCCGTGTTTA-CTCGAG-TAAACACGGAGCATCGATACT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGTATCGATGCTCCGTGTTTACTCGAGTAAACACGGAGCATCGATACTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGTATCGATGCTCCGTGTTTACTCGAGTAAACACGGAGCATCGATACT-3'

Other clones with same target sequence:

(none)