Construct: shRNA TRCN0000341949

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_010305.1-811s21c1
DNA Barcode:
TACCTTCAAAGATCTTCATTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Gnai1 (14677)
Gene Description:
guanine nucleotide binding protein (G protein), alpha inhibiting 1
Transcript:
RefSeq NM_010305.1 (CURRENT)
Match location:
Position 811 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000341949)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TACCTTCAAAGATCTTCATTT
Hairpin Sequence:
5'-CCGG-TACCTTCAAAGATCTTCATTT-CTCGAG-AAATGAAGATCTTTGAAGGTA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTACCTTCAAAGATCTTCATTTCTCGAGAAATGAAGATCTTTGAAGGTATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATACCTTCAAAGATCTTCATTTCTCGAGAAATGAAGATCTTTGAAGGTA-3'

Other clones with same target sequence:

(none)