Construct: shRNA TRCN0000341950

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_010305.1-1340s21c1
DNA Barcode:
CCAACAGATGAGTACTTATAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Gnai1 (14677)
Gene Description:
guanine nucleotide binding protein (G protein), alpha inhibiting 1
Transcript:
RefSeq NM_010305.1 (CURRENT)
Match location:
Position 1340 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000341950)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAACAGATGAGTACTTATAT
Hairpin Sequence:
5'-CCGG-CCAACAGATGAGTACTTATAT-CTCGAG-ATATAAGTACTCATCTGTTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAACAGATGAGTACTTATATCTCGAGATATAAGTACTCATCTGTTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAACAGATGAGTACTTATATCTCGAGATATAAGTACTCATCTGTTGG-3'

Other clones with same target sequence:

(none)