Construct: shRNA TRCN0000342152

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001163267.1-3049s21c1
DNA Barcode:
GGATGAAGCTTTGGGTATTAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Cfap58 (381229)
Gene Description:
cilia and flagella associated protein 58
Transcript:
RefSeq NM_001163267.1 (CURRENT)
Match location:
Position 3049 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000342152)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GGATGAAGCTTTGGGTATTAA
Hairpin Sequence:
5'-CCGG-GGATGAAGCTTTGGGTATTAA-CTCGAG-TTAATACCCAAAGCTTCATCC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGGATGAAGCTTTGGGTATTAACTCGAGTTAATACCCAAAGCTTCATCCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGGATGAAGCTTTGGGTATTAACTCGAGTTAATACCCAAAGCTTCATCC-3'

Other clones with same target sequence:

(none)