Construct: shRNA TRCN0000342205

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001163267.1-1549s21c1
DNA Barcode:
CCGAATCAGAGACGAAGTTAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Cfap58 (381229)
Gene Description:
cilia and flagella associated protein 58
Transcript:
RefSeq NM_001163267.1 (CURRENT)
Match location:
Position 1549 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000342205)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCGAATCAGAGACGAAGTTAA
Hairpin Sequence:
5'-CCGG-CCGAATCAGAGACGAAGTTAA-CTCGAG-TTAACTTCGTCTCTGATTCGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCGAATCAGAGACGAAGTTAACTCGAGTTAACTTCGTCTCTGATTCGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCGAATCAGAGACGAAGTTAACTCGAGTTAACTTCGTCTCTGATTCGG-3'

Other clones with same target sequence:

(none)