Construct: shRNA TRCN0000342472
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_000082.3-1239s21c1
- DNA Barcode:
- GCAGCAGTGATGAAGAAGGAT
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- ERCC8 (1161)
- Gene Description:
- ERCC excision repair 8, CSA ubiquitin ligase complex subunit
- Transcript:
- RefSeq NM_000082.3 (CURRENT)
- Match location:
- Position 1239 (CDS)
Vector Information:
- Vector Backbone:
- pLKO_005
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000342472)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- GCAGCAGTGATGAAGAAGGAT
- Hairpin Sequence:
-
5'-CCGG-GCAGCAGTGATGAAGAAGGAT-CTCGAG-ATCCTTCTTCATCACTGCTGC-TTTTTG-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGGCAGCAGTGATGAAGAAGGATCTCGAGATCCTTCTTCATCACTGCTGCTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAAGCAGCAGTGATGAAGAAGGATCTCGAGATCCTTCTTCATCACTGCTGC-3'
Other clones with same target sequence:
-
TRCN0000003719
