Construct: shRNA TRCN0000003719

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_000082.x-1205s1c1
DNA Barcode:
GCAGCAGTGATGAAGAAGGAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ERCC8 (1161)
Gene Description:
ERCC excision repair 8, CSA ubiquitin ligase complex subunit
Transcript:
RefSeq NM_000082.1 (NON-CURRENT)
Match location:
Position 1205 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000003719)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAGCAGTGATGAAGAAGGAT
Hairpin Sequence:
5'-CCGG-GCAGCAGTGATGAAGAAGGAT-CTCGAG-ATCCTTCTTCATCACTGCTGC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAGCAGTGATGAAGAAGGATCTCGAGATCCTTCTTCATCACTGCTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAGCAGTGATGAAGAAGGATCTCGAGATCCTTCTTCATCACTGCTGC-3'

Other clones with same target sequence:

  1. TRCN0000342472