Construct: shRNA TRCN0000343279

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_005721.3-663s21c1
DNA Barcode:
CCTCCATTGAATACTCCAGAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ACTR3 (10096)
Gene Description:
actin related protein 3
Transcript:
RefSeq NM_005721.3 (NON-CURRENT)
Match location:
Position 663 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000343279)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTCCATTGAATACTCCAGAA
Hairpin Sequence:
5'-CCGG-CCTCCATTGAATACTCCAGAA-CTCGAG-TTCTGGAGTATTCAATGGAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTCCATTGAATACTCCAGAACTCGAGTTCTGGAGTATTCAATGGAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTCCATTGAATACTCCAGAACTCGAGTTCTGGAGTATTCAATGGAGG-3'

Other clones with same target sequence:

  1. TRCN0000029379