Construct: shRNA TRCN0000343920

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001983.2-946s21c1
DNA Barcode:
CAAGAGAAGATCTGGCCTTAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ERCC1 (2067)
Gene Description:
ERCC excision repair 1, endonuclease non-catalytic subunit
Transcript:
RefSeq NM_001983.2 (NON-CURRENT)
Match location:
Position 946 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000343920)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAAGAGAAGATCTGGCCTTAT
Hairpin Sequence:
5'-CCGG-CAAGAGAAGATCTGGCCTTAT-CTCGAG-ATAAGGCCAGATCTTCTCTTG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAAGAGAAGATCTGGCCTTATCTCGAGATAAGGCCAGATCTTCTCTTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAAGAGAAGATCTGGCCTTATCTCGAGATAAGGCCAGATCTTCTCTTG-3'

Other clones with same target sequence:

  1. TRCN0000049920