Construct: shRNA TRCN0000345027

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_007395.3-304s21c1
DNA Barcode:
GCTGCTATATTGACTTCTGCA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Acvr1b (11479)
Gene Description:
activin A receptor, type 1B
Transcript:
RefSeq NM_007395.3 (CURRENT)
Match location:
Position 304 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000345027)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTGCTATATTGACTTCTGCA
Hairpin Sequence:
5'-CCGG-GCTGCTATATTGACTTCTGCA-CTCGAG-TGCAGAAGTCAATATAGCAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTGCTATATTGACTTCTGCACTCGAGTGCAGAAGTCAATATAGCAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTGCTATATTGACTTCTGCACTCGAGTGCAGAAGTCAATATAGCAGC-3'

Other clones with same target sequence:

  1. TRCN0000022577