Construct: shRNA TRCN0000345170

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_010765.2-1103s21c1
DNA Barcode:
GCACTGTCACTTGCTAAACAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Mapkapk5 (17165)
Gene Description:
MAP kinase-activated protein kinase 5
Transcript:
RefSeq NM_010765.2 (CURRENT)
Match location:
Position 1103 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000345170)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCACTGTCACTTGCTAAACAT
Hairpin Sequence:
5'-CCGG-GCACTGTCACTTGCTAAACAT-CTCGAG-ATGTTTAGCAAGTGACAGTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCACTGTCACTTGCTAAACATCTCGAGATGTTTAGCAAGTGACAGTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCACTGTCACTTGCTAAACATCTCGAGATGTTTAGCAAGTGACAGTGC-3'

Other clones with same target sequence:

  1. TRCN0000024198