Construct: shRNA TRCN0000345219

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_026917.5-550s21c1
DNA Barcode:
CCCAAAGGAAATGCCACTAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Zdhhc3 (69035)
Gene Description:
zinc finger, DHHC domain containing 3
Transcript:
RefSeq NM_026917.5 (CURRENT)
Match location:
Position 550 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000345219)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCAAAGGAAATGCCACTAAA
Hairpin Sequence:
5'-CCGG-CCCAAAGGAAATGCCACTAAA-CTCGAG-TTTAGTGGCATTTCCTTTGGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCAAAGGAAATGCCACTAAACTCGAGTTTAGTGGCATTTCCTTTGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCAAAGGAAATGCCACTAAACTCGAGTTTAGTGGCATTTCCTTTGGG-3'

Other clones with same target sequence:

  1. TRCN0000162755
  2. TRCN0000120664