Construct: shRNA TRCN0000345316

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_170757.1-2182s21c1
DNA Barcode:
GAGAATGGAAACCACGATAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ccdc186 (213993)
Gene Description:
coiled-coil domain containing 186
Transcript:
RefSeq NM_170757.1 (CURRENT)
Match location:
Position 2182 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000345316)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAGAATGGAAACCACGATAAA
Hairpin Sequence:
5'-CCGG-GAGAATGGAAACCACGATAAA-CTCGAG-TTTATCGTGGTTTCCATTCTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAGAATGGAAACCACGATAAACTCGAGTTTATCGTGGTTTCCATTCTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAGAATGGAAACCACGATAAACTCGAGTTTATCGTGGTTTCCATTCTC-3'

Other clones with same target sequence:

(none)