Construct: shRNA TRCN0000345561

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_027760.2-147s21c1
DNA Barcode:
CAGGAGGTTGTAATAGCCTTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Rassf8 (71323)
Gene Description:
Ras association (RalGDS/AF-6) domain family (N-terminal) member 8
Transcript:
RefSeq NM_027760.2 (CURRENT)
Match location:
Position 147 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000345561)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAGGAGGTTGTAATAGCCTTA
Hairpin Sequence:
5'-CCGG-CAGGAGGTTGTAATAGCCTTA-CTCGAG-TAAGGCTATTACAACCTCCTG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAGGAGGTTGTAATAGCCTTACTCGAGTAAGGCTATTACAACCTCCTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAGGAGGTTGTAATAGCCTTACTCGAGTAAGGCTATTACAACCTCCTG-3'

Other clones with same target sequence:

  1. TRCN0000121039