Construct: shRNA TRCN0000345968

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001164233.1-1684s21c1
DNA Barcode:
TGCTGATAATGAAGGTCAATA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Slco6d1 (70866)
Gene Description:
solute carrier organic anion transporter family, member 6d1
Transcript:
RefSeq NM_001164233.1 (CURRENT)
Match location:
Position 1684 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000345968)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGCTGATAATGAAGGTCAATA
Hairpin Sequence:
5'-CCGG-TGCTGATAATGAAGGTCAATA-CTCGAG-TATTGACCTTCATTATCAGCA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGCTGATAATGAAGGTCAATACTCGAGTATTGACCTTCATTATCAGCATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGCTGATAATGAAGGTCAATACTCGAGTATTGACCTTCATTATCAGCA-3'

Other clones with same target sequence:

(none)