Construct: shRNA TRCN0000346018

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001033372.2-860s21c1
DNA Barcode:
GAGTGTGATCCTGCTGTTACA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Gm648 (270599)
Gene Description:
predicted gene 648
Transcript:
RefSeq NM_001033372.2 (CURRENT)
Match location:
Position 860 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000346018)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAGTGTGATCCTGCTGTTACA
Hairpin Sequence:
5'-CCGG-GAGTGTGATCCTGCTGTTACA-CTCGAG-TGTAACAGCAGGATCACACTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAGTGTGATCCTGCTGTTACACTCGAGTGTAACAGCAGGATCACACTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAGTGTGATCCTGCTGTTACACTCGAGTGTAACAGCAGGATCACACTC-3'

Other clones with same target sequence:

(none)