Construct: shRNA TRCN0000346117

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001162940.1-161s21c1
DNA Barcode:
GGCTTCACACACCCATGTATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Olfr715b (384732)
Gene Description:
olfactory receptor 715B
Transcript:
RefSeq NM_001162940.1 (CURRENT)
Match location:
Position 161 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000346117)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GGCTTCACACACCCATGTATT
Hairpin Sequence:
5'-CCGG-GGCTTCACACACCCATGTATT-CTCGAG-AATACATGGGTGTGTGAAGCC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGGCTTCACACACCCATGTATTCTCGAGAATACATGGGTGTGTGAAGCCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGGCTTCACACACCCATGTATTCTCGAGAATACATGGGTGTGTGAAGCC-3'

Other clones with same target sequence:

(none)