Construct: shRNA TRCN0000346180

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_027556.1-7815s21c1
DNA Barcode:
GTGTAAATGACCTGCTTATAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Cep192 (70799)
Gene Description:
centrosomal protein 192
Transcript:
RefSeq NM_027556.1 (CURRENT)
Match location:
Position 7815 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000346180)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTGTAAATGACCTGCTTATAT
Hairpin Sequence:
5'-CCGG-GTGTAAATGACCTGCTTATAT-CTCGAG-ATATAAGCAGGTCATTTACAC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTGTAAATGACCTGCTTATATCTCGAGATATAAGCAGGTCATTTACACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTGTAAATGACCTGCTTATATCTCGAGATATAAGCAGGTCATTTACAC-3'

Other clones with same target sequence:

(none)