Construct: shRNA TRCN0000346211

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_027181.1-134s21c1
DNA Barcode:
AGAAGTCTCAGGGTCCTAAAG

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Pin4 (69713)
Gene Description:
protein (peptidyl-prolyl cis/trans isomerase) NIMA-interacting, 4 (parvulin)
Transcript:
RefSeq NM_027181.1 (CURRENT)
Match location:
Position 134 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000346211)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGAAGTCTCAGGGTCCTAAAG
Hairpin Sequence:
5'-CCGG-AGAAGTCTCAGGGTCCTAAAG-CTCGAG-CTTTAGGACCCTGAGACTTCT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGAAGTCTCAGGGTCCTAAAGCTCGAGCTTTAGGACCCTGAGACTTCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGAAGTCTCAGGGTCCTAAAGCTCGAGCTTTAGGACCCTGAGACTTCT-3'

Other clones with same target sequence:

(none)