Construct: shRNA TRCN0000346280

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001033306.1-1281s21c1
DNA Barcode:
ATGACTACTCCGATCCCTTTG

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Shb (230126)
Gene Description:
src homology 2 domain-containing transforming protein B
Transcript:
RefSeq NM_001033306.1 (CURRENT)
Match location:
Position 1281 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000346280)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ATGACTACTCCGATCCCTTTG
Hairpin Sequence:
5'-CCGG-ATGACTACTCCGATCCCTTTG-CTCGAG-CAAAGGGATCGGAGTAGTCAT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGATGACTACTCCGATCCCTTTGCTCGAGCAAAGGGATCGGAGTAGTCATTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAATGACTACTCCGATCCCTTTGCTCGAGCAAAGGGATCGGAGTAGTCAT-3'

Other clones with same target sequence:

(none)