Construct: shRNA TRCN0000346443

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_029859.1-977s21c1
DNA Barcode:
TTTGGTAGAGAGGGTCAAATC

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ccdc183 (77058)
Gene Description:
coiled-coil domain containing 183
Transcript:
RefSeq NM_029859.1 (CURRENT)
Match location:
Position 977 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000346443)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TTTGGTAGAGAGGGTCAAATC
Hairpin Sequence:
5'-CCGG-TTTGGTAGAGAGGGTCAAATC-CTCGAG-GATTTGACCCTCTCTACCAAA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTTTGGTAGAGAGGGTCAAATCCTCGAGGATTTGACCCTCTCTACCAAATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATTTGGTAGAGAGGGTCAAATCCTCGAGGATTTGACCCTCTCTACCAAA-3'

Other clones with same target sequence:

(none)