Construct: shRNA TRCN0000346626

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_153129.2-1231s21c1
DNA Barcode:
GCCCTCCTGAAACGATTCAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Pacs1 (107975)
Gene Description:
phosphofurin acidic cluster sorting protein 1
Transcript:
RefSeq NM_153129.2 (CURRENT)
Match location:
Position 1231 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000346626)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCCTCCTGAAACGATTCAAA
Hairpin Sequence:
5'-CCGG-GCCCTCCTGAAACGATTCAAA-CTCGAG-TTTGAATCGTTTCAGGAGGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCCTCCTGAAACGATTCAAACTCGAGTTTGAATCGTTTCAGGAGGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCCTCCTGAAACGATTCAAACTCGAGTTTGAATCGTTTCAGGAGGGC-3'

Other clones with same target sequence:

  1. TRCN0000190597