Construct: shRNA TRCN0000346872

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001163722.1-361s21c1
DNA Barcode:
GGAAGAAGCCTCATGCTATAG

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Smim1 (68859)
Gene Description:
small integral membrane protein 1
Transcript:
RefSeq NM_001163722.1 (CURRENT)
Match location:
Position 361 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000346872)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GGAAGAAGCCTCATGCTATAG
Hairpin Sequence:
5'-CCGG-GGAAGAAGCCTCATGCTATAG-CTCGAG-CTATAGCATGAGGCTTCTTCC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGGAAGAAGCCTCATGCTATAGCTCGAGCTATAGCATGAGGCTTCTTCCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGGAAGAAGCCTCATGCTATAGCTCGAGCTATAGCATGAGGCTTCTTCC-3'

Other clones with same target sequence:

(none)