Construct: shRNA TRCN0000346974

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_175258.3-1629s21c1
DNA Barcode:
CGGATTGTACTCTTATGGGTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Rapgef6 (192786)
Gene Description:
Rap guanine nucleotide exchange factor (GEF) 6
Transcript:
RefSeq NM_175258.3 (NON-CURRENT)
Match location:
Position 1629 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000346974)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGGATTGTACTCTTATGGGTA
Hairpin Sequence:
5'-CCGG-CGGATTGTACTCTTATGGGTA-CTCGAG-TACCCATAAGAGTACAATCCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGGATTGTACTCTTATGGGTACTCGAGTACCCATAAGAGTACAATCCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGGATTGTACTCTTATGGGTACTCGAGTACCCATAAGAGTACAATCCG-3'

Other clones with same target sequence:

  1. TRCN0000177845