Construct: shRNA TRCN0000347708

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001081471.1-536s21c1
DNA Barcode:
GAACGGTCTACCTGGAATTAC

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Mettl7a3 (668178)
Gene Description:
methyltransferase like 7A3
Transcript:
RefSeq NM_001081471.1 (CURRENT)
Match location:
Position 536 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000347708)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAACGGTCTACCTGGAATTAC
Hairpin Sequence:
5'-CCGG-GAACGGTCTACCTGGAATTAC-CTCGAG-GTAATTCCAGGTAGACCGTTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAACGGTCTACCTGGAATTACCTCGAGGTAATTCCAGGTAGACCGTTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAACGGTCTACCTGGAATTACCTCGAGGTAATTCCAGGTAGACCGTTC-3'

Other clones with same target sequence:

(none)