Construct: shRNA TRCN0000347986

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_008250.2-1118s21c1
DNA Barcode:
CCCTATGCTGTGCTCACTAAG

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Hlx (15284)
Gene Description:
H2.0-like homeobox
Transcript:
RefSeq NM_008250.2 (CURRENT)
Match location:
Position 1118 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000347986)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCTATGCTGTGCTCACTAAG
Hairpin Sequence:
5'-CCGG-CCCTATGCTGTGCTCACTAAG-CTCGAG-CTTAGTGAGCACAGCATAGGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCTATGCTGTGCTCACTAAGCTCGAGCTTAGTGAGCACAGCATAGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCTATGCTGTGCTCACTAAGCTCGAGCTTAGTGAGCACAGCATAGGG-3'

Other clones with same target sequence:

(none)