Construct: shRNA TRCN0000348455

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_198710.3-766s21c1
DNA Barcode:
AGCTTACACAGTCCGTCAAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Sypl (19027)
Gene Description:
synaptophysin-like protein
Transcript:
RefSeq NM_198710.3 (CURRENT)
Match location:
Position 766 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000348455)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGCTTACACAGTCCGTCAAAT
Hairpin Sequence:
5'-CCGG-AGCTTACACAGTCCGTCAAAT-CTCGAG-ATTTGACGGACTGTGTAAGCT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGCTTACACAGTCCGTCAAATCTCGAGATTTGACGGACTGTGTAAGCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGCTTACACAGTCCGTCAAATCTCGAGATTTGACGGACTGTGTAAGCT-3'

Other clones with same target sequence:

(none)